Coding

Part:BBa_K4234000

Designed by: Robert Kaptur   Group: iGEM22_Duke   (2022-10-11)


IDH1 R132H mutation

This part contains the coding region of the IDH1 enzyme with a R132H mutation. IDH1 codes for isocitrate dehydrogenase 1, which catalyzing the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. The mutant form of the enzyme catalyzes the production of D-2-hydroxyglutarate. The R132H variant of IDH1 is known to be prevalent in gliomas and glioblastomas, providing a good target for our analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 253
    Illegal BglII site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 253
    Illegal NgoMIV site found at 1105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 970
    Illegal BsaI.rc site found at 1093
    Illegal SapI.rc site found at 777


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Parameters
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