Composite

Part:BBa_K4241801

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-11)
Revision as of 02:06, 12 October 2022 by DaLegends6 (Talk | contribs)


CvNDI1_Ulp1_TRAmy3D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1376
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849

Overview

This composite part is an inducible high-expression system to produce Ulp1 for SUMO-cleavage in microalgae. The expression of this part is triggered by nitrogen starvation.

Usage and Biology

This part is used for the inducible expression of Ulp1 in a high-expression manner. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.

Parts that comprise this composite component

Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
Ulp1_Active Domain (Microalgae) - BBa_K4241797
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794



[edit]
Categories
Parameters
None