Part:BBa_K4241801

CvNDI1_Ulp1_TRAmy3D

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 2058
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  Illegal PstI site found at 1242
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 2058
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  Illegal PstI site found at 1242
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1376
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 2058
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  Illegal PstI site found at 1242
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 2058
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  Illegal PstI site found at 1242
  Illegal NgoMIV site found at 264
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 849
  

Overview

This composite part is an inducible high-expression system to produce Ulp1 for SUMO-cleavage in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

This part is used for the inducible expression of Ulp1 in a high-expression manner. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.

Parts that comprise this composite component

Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
Ulp1_Active Domain (Microalgae) - BBa_K4241797
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9