Reporter
Part:BBa_K4365001
Designed by: Giorgio Gilioli Group: iGEM22_TU_Dresden (2022-09-29)
Revision as of 14:32, 11 October 2022 by Svetlana ia (Talk | contribs)
turboRFP
Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or for protein detection if fused with another protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
TurboRFP is a red-orange fluorescent protein derived from sea anemone Entacmaea quadricolor [1]. Possessing prolonged fluorescence lifetime and high pH-stability.and high pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in mammalian cells as early as within 8-10 hrs after transfection [1].
- ↑ 1.0 1.1 Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062
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Categories
Parameters
| None |