Part:BBa_K4365001

turboRFP

Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or for protein detection if fused with another protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

Figure 1. Picture of the sea anemone Entacmaea quadricolor. Image from Wikipedia.org

TurboRFP is a red-orange fluorescent protein derived from sea anemone Entacmaea quadricolor ^[1]. It possesses prolonged fluorescence lifetime and high pH-stability, is fast-maturing, and is more than twice brighter than DsRed2. ^[1]

Figure 2. Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. 187 % brighter than of EGFP. FP database

Figure 3. Fluorescent bacterial colonies with mRFP1 and turboRFP1 under transilluminator.

↑ ^1.0 ^1.1 Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062

[ameno-1] 1.0 ^1.1 Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062

[1]