Other
DsbA
Part:BBa_K243002:Design
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-08)
DsbA signal sequence (enables periplasm export)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the signal sequence had to be located at the N-terminus of the (fusion)protein.
Source
Sequence of the DsbA was copied out from E.coli B genom Synthesized oligos with signal sequence of DsbA by sigma.
References
Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display NATURE BIOTECHNOLOGY Vol.24 Issue:7 Pages: 823-831