Other
DsbA

Part:BBa_K243002:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-08)

DsbA signal sequence (enables periplasm export)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal sequence has to be located at the N-terminus of the fused protein. Designed according to RFC 25.
Commented GenBank file

Source

The amino acid sequence of the DsbA signal sequence was extracted from the E. coli B genome. Synthesized oligos encoding the signal sequence of DsbA were designed and eventually ordered from Sigma.

References

Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display Nature Biotechnology Vol.24 Issue:7 Pages: 823-831