Composite

Part:BBa_K4164009:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)
Revision as of 08:06, 11 October 2022 by JieYang (Talk | contribs)

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constitutive expression of FXR-ddRFPA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1122


Design Notes

We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and FXR-ddRFPA1(BBa_K4164994) by homologous recombination.


Source

This sequence is composed of BBa_J23105, BBa_B0034 and BBa_K416994.

References