Composite

Part:BBa_K4156127

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-10)
Revision as of 14:35, 10 October 2022 by Registry (Talk | contribs)

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HlpA with INP-mRFP

HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.We added the red fluorescent protein after the HlpA gene for subsequent visualization of bacterial adhesion experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 194
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 527
    Illegal AgeI site found at 855
    Illegal AgeI site found at 1508
    Illegal AgeI site found at 1620
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None