Part:BBa_K4156127
HlpA with INP-mRFP
HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.We added the red fluorescent protein after the HlpA gene for subsequent visualization of bacterial adhesion experiments.
Characterization
Enhancement of E. coli EcN tropism for colorectal cancer
To make the engineered strain nengg better adhere to colorectal cancer cells, we used engineered bacteria carrying the adhesion proteins INP-HlpA and mRFP, and the fluorescence was significantly enhanced compared to the control engineered bacteria expressing mRFP alone, indicating that the HlpA protein could effectively improve the adhesion of EcN to colorectal cells. (where CT26 and RKO were magnified 400-fold, while RKO was magnified 300-fold), and this change was particularly evident in RKO cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 452
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 194
Illegal NgoMIV site found at 276
Illegal NgoMIV site found at 527
Illegal AgeI site found at 855
Illegal AgeI site found at 1508
Illegal AgeI site found at 1620 - 1000COMPATIBLE WITH RFC[1000]
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