Part:BBa_K4375013

Myc epitope tag with SphI restriction site

A myc tag is a polypeptide protein tag derived from the c-myc gene, and we appended a SpHI restriction site to its N-terminal.

Usage and Biology

The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor. A Myc-tag can be used to detect expression of recombinant proteins. First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Leo JC, Oberhettinger P, Schütz M, Linke D. The inverse autotransporter family: intimin, invasin and related proteins. International Journal of Medical Microbiology. 2015 Feb 1;305(2):276-82.

Wentzel A, Christmann A, Adams T, Kolmar H. Display of passenger proteins on the surface of Escherichia coli K-12 by the enterohemorrhagic E. coli intimin EaeA. Journal of bacteriology. 2001 Dec 15;183(24):7273-84.

Rutherford N, Mourez M. Surface display of proteins by Gram-negative bacterial autotransporters. Microbial cell factories. 2006 Dec;5(1):1-5.

Salema V, Marín E, Martínez-Arteaga R, Ruano-Gallego D, Fraile S, Margolles Y, Teira X, Gutierrez C, Bodelón G, Fernández LÁ. Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies. PloS one. 2013 Sep 23;8(9):e75126.