Reporter

Part:BBa_K4245132:Design

Designed by: Fulin Zhou, Baoxin Li, and Jiyuan Maa   Group: iGEM22_Lambert_GA   (2022-09-23)
Revision as of 19:53, 9 October 2022 by Manasvigupta (Talk | contribs)


Black Hole Quencher 1 (BHQ1) DNA Probe


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Ogawa, M., Kosaka, N., Longmire, M. R., Urano, Y., Choyke, P. L., & Kobayashi, H. (2009). Fluorophore-quencher based activatable targeted optical probes for detecting in vivo cancer metastases. Molecular Pharmaceutics, 6(2), 386–395. https://doi.org/10.1021/mp800115t

Zhou, F., Li, B., & Ma, J. (2014). A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification. RSC Advances, 5(6), 4019–4025. https://doi.org/10.1039/c4ra14467h