Reporter

Part:BBa_K4245132:Design

Designed by: Fulin Zhou, Baoxin Li, and Jiyuan Maa   Group: iGEM22_Lambert_GA   (2022-09-23)


Black Hole Quencher 1 (BHQ1) DNA Probe


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed by researchers of Key Lab at Shaanxi Normal University (Zhou et. al, 2014).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Ogawa, M., Kosaka, N., Longmire, M. R., Urano, Y., Choyke, P. L., & Kobayashi, H. (2009). Fluorophore-quencher based activatable targeted optical probes for detecting in vivo cancer metastases. Molecular Pharmaceutics, 6(2), 386–395. https://doi.org/10.1021/mp800115t

Zhou, F., Li, B., & Ma, J. (2014). A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification. RSC Advances, 5(6), 4019–4025. https://doi.org/10.1039/c4ra14467h