Coding

Part:BBa_K4214002

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)
Revision as of 12:42, 9 October 2022 by Zeniazen5 (Talk | contribs)


hGIF with 6xHis-tag

Human GIF with His6-tag. This part has the native signal peptide removed, as it is not required for bacterial expression or in our mammalian expression vector. This part was used to produce and purify our Human GIF in bacteria, through the composite parts BBa_K421403 (with LacI promotor) and BBa_K421404 (with LacI promotor and pelB leader sequence).

Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • This part of hGIF does not contain the native signal peptide (AA1-18)
  • This part was used in parts BBa_K421403andBBa_K421404


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 257
    Illegal BsaI.rc site found at 937
    Illegal SapI site found at 927


[edit]
Categories
Parameters
None