User contributions
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- 14:35, 9 October 2022 (diff | hist) . . (+612) . . Part:BBa K4214003
- 14:32, 9 October 2022 (diff | hist) . . (+352) . . N File:BBa K4214003 Gel.png (Restriction Digestion of the pSB1C3 with LacI [https://parts.igem.org/Part:BBa_J04500 (BBa_J04500)] using the SpeI and PstI restriction enzymes and restriction digestion of the Human GIF with His6-tag (BBa_K4214002)vby using the XbaI and PstI. After the..) (current)
- 14:12, 9 October 2022 (diff | hist) . . (+43) . . Part:BBa K4214004 (current)
- 14:07, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214004
- 14:06, 9 October 2022 (diff | hist) . . (+615) . . Part:BBa K4214004
- 13:38, 9 October 2022 (diff | hist) . . (+53) . . Part:BBa K4214003
- 13:36, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214003
- 13:36, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214003
- 13:36, 9 October 2022 (diff | hist) . . (+1,611) . . Part:BBa K4214003
- 13:33, 9 October 2022 (diff | hist) . . (+155) . . N File:WB BB12.png (Western blot image from the protein production of wtIF in the pSB1C3 plasmid with the LacI promotor after IPTG induction. wtIF is detected around 55-70 kDa) (current)
- 13:27, 9 October 2022 (diff | hist) . . (-3) . . Part:BBa K4214003
- 13:27, 9 October 2022 (diff | hist) . . (-25) . . Part:BBa K4214003
- 13:26, 9 October 2022 (diff | hist) . . (-76) . . Part:BBa K4214003
- 13:26, 9 October 2022 (diff | hist) . . (+28) . . Part:BBa K4214003
- 13:25, 9 October 2022 (diff | hist) . . (+104) . . Part:BBa K4214003
- 13:25, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214003
- 13:24, 9 October 2022 (diff | hist) . . (-9) . . Part:BBa K4214003
- 13:21, 9 October 2022 (diff | hist) . . (+330) . . N File:BBa K4214003 SDSPAGE.png (A production culture was set up using BL21 ''E. coli'' and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt...) (current)
- 13:20, 9 October 2022 (diff | hist) . . (+124) . . Part:BBa K4214003
- 13:19, 9 October 2022 (diff | hist) . . (+839) . . Part:BBa K4214003
- 12:52, 9 October 2022 (diff | hist) . . (+5) . . Part:BBa K4214000
- 12:52, 9 October 2022 (diff | hist) . . (+14) . . Part:BBa K4214002 (current)
- 12:51, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214002
- 12:50, 9 October 2022 (diff | hist) . . (-1) . . Part:BBa K4214002
- 12:50, 9 October 2022 (diff | hist) . . (+186) . . Part:BBa K4214002
- 12:42, 9 October 2022 (diff | hist) . . (+1) . . Part:BBa K4214002
- 12:42, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214002
- 12:42, 9 October 2022 (diff | hist) . . (-2) . . Part:BBa K4214002
- 12:42, 9 October 2022 (diff | hist) . . (-1) . . Part:BBa K4214002
- 12:41, 9 October 2022 (diff | hist) . . (+12) . . Part:BBa K4214002
- 12:40, 9 October 2022 (diff | hist) . . (-24) . . Part:BBa K4214002
- 12:15, 9 October 2022 (diff | hist) . . (+15) . . Part:BBa K4214002
- 12:09, 9 October 2022 (diff | hist) . . (+389) . . Part:BBa K4214002
- 10:55, 9 October 2022 (diff | hist) . . (+1) . . Part:BBa K4214000
- 10:40, 9 October 2022 (diff | hist) . . (-1) . . Part:BBa K4214000
- 10:40, 9 October 2022 (diff | hist) . . (-1) . . Part:BBa K4214000
- 10:40, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214000
- 10:39, 9 October 2022 (diff | hist) . . (+2) . . Part:BBa K4214000
- 10:39, 9 October 2022 (diff | hist) . . (-86) . . Part:BBa K4214000
- 10:31, 9 October 2022 (diff | hist) . . (+1,389) . . Part:BBa K4214000
- 10:25, 9 October 2022 (diff | hist) . . (+456) . . N File:FM.png (Fluorescence microscope pictures of HEK 293T cells [A to F]. Scale is marked at the bottom left of the image. A: Negative control in Bright field B: Negative control overlapped with Bright field and DAPI staining, C: Positive control in GFP channel, D:...) (current)
- 10:21, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4214000
- 10:21, 9 October 2022 (diff | hist) . . (+51) . . Part:BBa K4214000
- 10:20, 9 October 2022 (diff | hist) . . (+20) . . Part:BBa K4214000
- 10:17, 9 October 2022 (diff | hist) . . (+51) . . Part:BBa K4214000
- 10:16, 9 October 2022 (diff | hist) . . (+223) . . Part:BBa K4214000
- 10:14, 9 October 2022 (diff | hist) . . (+162) . . N File:BBa K4214000 Gel.png (Agarose gel image showing the results from restriction digestion of BBa_K4214000 with Bgl-II and SalI in order to continue with ligation to our expression vector.) (current)
- 10:12, 9 October 2022 (diff | hist) . . (+5) . . Part:BBa K4214000
- 10:12, 9 October 2022 (diff | hist) . . (-2) . . Part:BBa K4214000
- 10:10, 9 October 2022 (diff | hist) . . (-1,604) . . Part:BBa K4214000
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