Regulatory

Part:BBa_K4156123

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 03:00, 9 October 2022 by Hz (Talk | contribs)


OR gate

OR gate is a type of Boolean logic gate that can be used to activate downstream gene expression in response to an upstream counterpart promoter in the presence of any or all markers.


Usage and Biology

The OR gate is constructed with a p7 promoter and a terminator ( BBa_B0015 ). We added all cis Bxb1 integrase and Tp901 integrase sites on both sides of the terminator to form the so-called OR gate. Finally, we introduced RiboJ and BCD2 sequences to ensure smooth transcription with the OR gate on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 306


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Categories
Parameters
None