Coding

Part:BBa_K4197019

Designed by: Guillaume Gomez   Group: iGEM22_Toulouse_INSA-UPS   (2022-09-20)
Revision as of 22:25, 8 October 2022 by Charline baraban (Talk | contribs)


DARPin e2_79

Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE (Baumann et al., 2010).


Introduction

This part is composed of the gene coding for the DARPin E2_79 protein. DARPin is a protein that recognizes the constant part of IgE (Baumann et al., 2010). It has already been expressed in E. coli

Construction

The objective of the INSA-UPS 2022 team was to display DARPin E2_79 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K1694011).

References

  1. Baumann, M. J., Eggel, A., Amstutz, P., Stadler, B. M., & Vogel, M. (2010). DARPins against a functional IgE epitope. Immunology Letters, 133(2), 78–84. https://doi.org/10.1016/j.imlet.2010.07.005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None