Part:BBa_K4197019

DARPin e2_79

Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE (Baumann et al., 2010).

Introduction

This part is composed of the gene coding for the DARPin E2_79 protein. DARPin is a protein that recognizes the constant part of IgE (Baumann et al., 2010). It has already been expressed in E. coli

Construction

The objective of the INSA-UPS 2022 team was to display DARPin E2_79 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K1694011).

References

More information about the project for which the part was created: DAISY (INSA-UPS 2022)

Other parts to display allergens:

Other parts using the DARPin:
- OmpA_DARPin
- OmpA_DARPin_sfGFP
- OmpA_DARPin fusion + ihfB800-mTagBFP

1. Baumann, M. J., Eggel, A., Amstutz, P., Stadler, B. M., & Vogel, M. (2010). DARPins against a functional IgE epitope. Immunology Letters, 133(2), 78–84. https://doi.org/10.1016/j.imlet.2010.07.005

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 205
  
• 1000
  COMPATIBLE WITH RFC[1000]