Composite

Part:BBa_K4345017:Design

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 13:54, 8 October 2022 by LVdB (Talk | contribs) (→‎Design Notes)


pSynSense2.5000 PfdeAR-FdeR biosensor circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal NheI site found at 1523
    Illegal NotI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal BamHI site found at 1073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 870
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1544


Source

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419