Composite
Part:BBa_K4345017:Design
Designed by: Michelle Patricia Group: iGEM22_KU_Leuven (2022-09-30)
pSynSense2.5000 PfdeAR-FdeR biosensor circuit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 580
Illegal EcoRI site found at 1058
Illegal XbaI site found at 1088 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 580
Illegal EcoRI site found at 1058
Illegal NheI site found at 1523
Illegal NotI site found at 1094 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 580
Illegal EcoRI site found at 1058
Illegal BamHI site found at 1073 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 580
Illegal EcoRI site found at 1058
Illegal XbaI site found at 1088 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 580
Illegal EcoRI site found at 1058
Illegal XbaI site found at 1088
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 779
Illegal NgoMIV site found at 870 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1544
Source
Herbaspirillum seropedicae
References
De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419