Composite
Part:BBa_K4368003:Design
Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez Group: iGEM22_UMA_MALAGA (2022-09-26)
pcstA + rbs + bglX + terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1609
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1457
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1367
Design Notes
Consideration should be given to the correct optimization of the sequence for use in different organisms.
Source
The bglX gene is an optimized synthetic construction based on: BBa_K4368002.