Coding

Part:BBa_K4368002

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)

bglX

Description

BglX (BBa_K523002) encodes for the β-glucosidase gene of Escherichia coli. This enzyme is responsible of the degradation of cellulose working coordinated with the genes cenA and cex. This complex is known as CAZymes. In detail, bglX degradates the cellobiose formed by cenA and cex and transformed it into glucose. This basic part only corresponds to the coding sequence of the gene, no RBS or terminator is attached.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1207

Contribution


[edit]
Categories
Parameters
None