Plasmid

Part:BBa_K4298012

Designed by: Masixuan Zhao   Group: iGEM22_Peking   (2022-09-30)
Revision as of 06:09, 7 October 2022 by GrizzlyZhao (Talk | contribs) (test update)


pUC19-ITR-EPI

PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1000
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505


[edit]
Categories
//chassis/eukaryote/yeast
//plasmid
Parameters
None