Plasmid
Part:BBa_K4298012
Designed by: Masixuan Zhao Group: iGEM22_Peking (2022-09-30)
pUC19-ITR-EPI
pUC19 plasmid, containing flanked inverted repeats and the insertion sequence is EPI with α-secretion signal. EPI between the inverted repeat can be introduced into P1 plasmid by homologous recombination at both ends.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1182
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1000
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 505
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
//plasmid
//plasmid
None |