Part:BBa_K4388003
Vitis vinifera Stilbene Synthase Mutant (VvSTS T50I/V170A)
The Vitis vinifera stilbene synthase (VvSTS) is an enzyme that catalyses the formation of a linear tetraketide intermediate from one 4-coumaroyl-CoA and three malonyl-CoA molecules, followed by cyclisation, decarboxylation, and dehydration reactions to form resveratrol.
STS is a key enzyme used for the production of resveratrol and pterostilbene. This version of the enzyme is a mutant with amino acid changes T50I and V170A. These mutations have been shown to increase catalytic efficiency from 3.29 (wild-type) to 4.29 μmol/h/g (Yan et al., 2021).
The ORF of this compound was obtained from NCBI GenBank code: DQ459351. This sequence was then codon optimized for E. coli K12, mutated at the desired positions, and two stop codons (TAATAA) were added to terminate translation.
Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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