Part:BBa_K4388003
Vitis vinifera Stilbene Synthase Mutant (VvSTS T50I/V170A)
The Vitis vinifera stilbene synthase (VvSTS) is an enzyme that catalyses the formation of a linear tetraketide intermediate from one 4-coumaroyl-CoA and three malonyl-CoA molecules, followed by cyclisation, decarboxylation, and dehydration reactions to form resveratrol.
STS is a key enzyme used for the production of resveratrol and pterostilbene. This version of the enzyme is a mutant with amino acid changes T50I and V170A. These mutations have been shown to increase catalytic efficiency from 3.29 (wild-type) to 4.29 μmol/h/g (Yan et al., 2021).
The ORF of this compound was obtained from NCBI GenBank code: DQ459351. This sequence was then codon optimized for E. coli K12, mutated at the desired positions, and two stop codons (TAATAA) were added to terminate translation.
The wild type version if this part is reported on the page: https://parts.igem.org/Part:BBa_K1033002
This part has been successfully ligated into a transcriptional unit: https://parts.igem.org/Part:BBa_K4388007:Experience
Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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