Reporter

Part:BBa_K259006

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-09-09)
Revision as of 18:53, 9 September 2009 by Pm5527 (Talk | contribs) (GFP Structure)

GFP-Terminator

GFP Structure

[Image:BCCS_600px-GFP_structure.png|center|500px|frame|The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB]

Part Description

This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.

Green Fluorescent protein (originally from part BBa_E0040)will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.

Table of Statistics

Chassis Monomer/Multimer Aminoacid Length/Weight Localisation Structure Excitation wavelengths Emmision wavelengths
Prokaryotic and Eukaryotic Bipartite 274 aminoacids beta-barrel(for GFP) hairpins for Terminator 501nm 511nm


References

<biblio>

  1. Valdivia pmid=8707053

</biblio>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter
//function/reporter/fluorescence
//terminator/double
Parameters
biologyT7TE-LuxIA
directionBidirectional
emission
emit511
excitation
excite501
forward_efficiency0.604[CC]/0.96[JK]
lum
proteinGFPmut3b
reversed_version2145
reverse_efficiency0.86[JK]
tagNone