Part:BBa_K259006

GFP-Terminator

This is a composite part made from the reporter gene GFP and a added on to a double terminator. It is ideal for adding on at the end of your constructs. See details below.

GFP Structure

The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB

Terminator Structure

The structure/sequence of the double terminator. Image from the original part page

Part Description

This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.

Green Fluorescent protein (originally from part BBa_E0040)will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.

Table of Statistics

Chassis	Monomer/Multimer	Aminoacid Length/Weight	Localisation	Structure	Excitation wavelengths	Emmision wavelengths
Prokaryotic and Eukaryotic	Bipartite	274 aminoacids		beta-barrel(for GFP) hairpins for Terminator	501nm	511nm

References

<biblio>

1. Valdivia pmid=8707053

</biblio>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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