Plasmid

Part:BBa_K4298012

Designed by: Masixuan Zhao   Group: iGEM22_Peking   (2022-09-30)
Revision as of 05:28, 4 October 2022 by Teddy Huang (Talk | contribs) (test update main)


pUC19-ITR-EPI

PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.

The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1000
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505


[edit]
Categories
//chassis/eukaryote/yeast
//plasmid
Parameters
None