Composite

Part:BBa_K4115044

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 06:45, 3 October 2022 by Yurj (Talk | contribs)


J23101-B0034-tagRFP-B0015

CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
Function gene reporter
Use in E.coli
RFC standard RFC 10, RFC 10 compatible
Backbone pBBR1
Submitted by (ShanghaiTech_China)

This composite part is used to express the tagRFP(BBa_K4115013) by a constitutive promoter J23101(J23101), thus we use red fluorescence as a detection.

[[File:TagRFP in gel beads.jpg]

Proof of function

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695
    Illegal SapI.rc site found at 77


[edit]
Categories
Parameters
None