Coding

Part:BBa_K4380011:Design

Designed by: Brigita Duchovska   Group: iGEM22_Vilnius-Lithuania   (2022-10-01)
Revision as of 21:07, 1 October 2022 by Brigita (Talk | contribs) (→‎Design Notes)


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence.

Source

Synthetic

References