Coding

Part:BBa_K4380011:Design

Designed by: Brigita Duchovska   Group: iGEM22_Vilnius-Lithuania   (2022-10-01)


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

eGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. eGFP (Enhanced green fluorescent protein) is used as a reporter protein to monitor the anchor peptide binding on polymer surfaces.

Source

Aequorea victoria, Zhang et al., 1996[1].

References

[1] Zhang, G., Gurtu, V., & Kain, S. R. (1996). An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochemical and Biophysical Research Communications, 227(3), 707–711. https://doi.org/10.1006/bbrc.1996.1573