Protein_Domain
S-tag

Part:BBa_T2015

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-09-02)
Revision as of 20:46, 2 September 2009 by Rshetty (Talk | contribs) (→‎Usage and Biology)

S-tag tail domain (KETAAAKFERQHMDS)

This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Usage and Biology

The S-tag binds to the S-fragment of RNase A and is eluted at low pH. An RNase A assay is possible for a quantitative assay of protein expression levels. Colorimetric assays are also available for detection of S-tagged proteins without the use of an antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 10
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


[edit]
Categories
//proteindomain/affinity
//proteindomain/tail
Parameters
None