Part:BBa_K4212001
CotG_3Gx3_ChiS
This part codes for a fusion protein consisting of the B. subtilis native CotG anchor protein and a B. pumilus chitinase enzyme. The CotG protein has a midspore localization and high abundance in B. subtilis spores, enabling spore display. In this case with the chitinase fusion, spores will display chitinases on the surface capable of breaking down chitin into its monomer components, such as N-acetylglucosamine. In the context of our project, the chitinase activity enables us to equip spores with the ability to breakdown the chitin-rich outer coat of fungal pathogens, not only inhibiting their activity but generating chitin monomers that can function as a biomarker, as well as an inducer of plant systemic immune response. This fusion includes a flexible linker, to reduce steric hindrance between the two protein domains and enable greater chitinase activity. The same was selected for testing following dry lab modelling.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 885
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 885
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 885
Illegal BamHI site found at 870 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 885
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 885
- 1000COMPATIBLE WITH RFC[1000]
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