Regulatory

Part:BBa_K4226003

Designed by: Siyu Han   Group: iGEM22_CAFA_China   (2022-09-19)
Revision as of 10:30, 29 September 2022 by Tigress K (Talk | contribs)


hrpL Promoter

This part is an improvement for the part：Promoter hrpL uploaded by iGEM13_HIT-Harbin. This promoter will be active, when both hrpR (hrpR Gene) and hrpS gene (hrpS Gene) are present. The hrpL Promoter is transcriptionally upregulated by hrpR and hrpS. The Amp30E Amplification Device consists of hrpR (hrpR Gene), hrpS (hrpS Gene) and hrpL promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.

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Categories
Parameters
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