Coding

Part:BBa_K4226001

Designed by: Siyu Han   Group: iGEM22_CAFA_China   (2022-09-19)


hrpR Gene

This part is an improvement for the part：HrpR Gene uploaded by iGEM13_HIT-Harbin. The hrpR gene codes for hrpR protein. hrpR protein binds to hrpS protein forming a complex, and the hrpL Promoter is transcriptionally upregulated by hrpR and hrpS Gene. The Amp30E Amplification Device consists of hrpR, hrpS Gene and hrpL Promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.

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Categories
Parameters
None