Composite
Part:BBa_K4408006
Designed by: Leyu Xu Group: iGEM22_Worldshaper-NJBIOX (2022-09-29)
hbd modified by oligopeptide SpyTag
This part is the coding region of hbd enzyme combined with genes coding for SpyTag oligopeptide and Linker 1. It is constructed by hbd (BBa_M1350), SpyTag(BBa_3329001) and linker 1(BBa_K4408003) sequences. It can be bounded with SpyCatcher-Linker 1-thl (BBa_4408005) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |