RBS

Part:BBa_K239008:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-08-17)
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RBS from RpoS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed.


Source

Genomic sequence from biocyc.org database. Escherichia coli K-12 substr. MG1655 Native unmodified RBS for RpoS, but RpoS transcription can be initiated at more than one position. Hence other native RBS are also possible.

References