RBS

Part:BBa_K239008:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-08-17)

RBS from RpoS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed.


Source

Genomic sequence from biocyc.org database. Escherichia coli K-12 substr. MG1655 Native unmodified RBS for RpoS, but RpoS transcription can be initiated at more than one position. Hence other native RBS are also possible.

References

Brown, L. et al. (2002) DksA affects ppGpp induction of RpoS at a translational level. J. Bacteriol. 184, 4455–4465