Coding

Part:BBa_K4202004:Design

Designed by: JUNYI LIU   Group: iGEM22_ZJU-China   (2022-09-25)
Revision as of 11:50, 25 September 2022 by Eeeeeartha (Talk | contribs)


TSLV-BS-CA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 540
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535


Design Notes

Replacing codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are more compatible with the codon usage bias of Bacillus subtilis, avoiding the emergence of rare codons.


Source

This part is created by codon optimization for Bacillus subtilis from Part:<a href="https://parts.igem.org/Part:BBa_K2232000:Design" target="_blank" rel="noopener noreferrer">BBa_K2232000</a>.

References

Faridi, S., & Satyanarayana, T. (2016). Characteristics of recombinant α-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1. International journal of biological macromolecules, 89, 659-668.