Coding
Part:BBa_K2232000:Design
Designed by: Wenkai Hu Group: iGEM17_SZU-China (2017-10-17)
TSLV1-CA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 540
Illegal NotI site found at 827 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 520
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 65
Illegal SapI.rc site found at 223
Illegal SapI.rc site found at 535
Design Notes
We use it as a coding protein for carbonic anhydrase
Source
GenBank: KR347171.1
References
Faridi S, Satyanarayana T. Characteristics of recombinant α-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1[J]. International Journal of Biological Macromolecules, 2016, 89:659-668.