Composite
Part:BBa_K4430010
Designed by: Ruyi Shi Group: iGEM22_GYHS (2022-09-20)
GFP-TFP
It is the key part that is responsible for expressing green fluorescent protein (GFP) fusion protein of tail fiber protein (TFP). TFP is a receptor binding protein (RBP) from the genome of E. coli phage T7. GFP emits green fluorescence under the confocal laser scanning microscope. Under such microscope, green fluorescence on the cell surface of E.coli after incubation with GFP-TFP proteins indicates specific binding of the proteins to the bacteria. We have demonstrated that GFP-TFP can bind strongly to the cell surfaces of E. coli strains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2201
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2169
Illegal AgeI site found at 1824
Illegal AgeI site found at 2262 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Illegal BsaI.rc site found at 1263
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Categories
Parameters
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