Composite

Part:BBa_K4430010

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)
Revision as of 02:10, 21 September 2022 by Registry (Talk | contribs)

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GFP-TFP

It is the key part that is responsible for expressing green fluorescent protein (GFP) fusion protein of tail fiber protein (TFP). TFP is a receptor binding protein (RBP) from the genome of E. coli phage T7. GFP emits green fluorescence under the confocal laser scanning microscope. Under such microscope, green fluorescence on the cell surface of E.coli after incubation with GFP-TFP proteins indicates specific binding of the proteins to the bacteria. We have demonstrated that GFP-TFP can bind strongly to the cell surfaces of E. coli strains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2201
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2169
    Illegal AgeI site found at 1824
    Illegal AgeI site found at 2262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641
    Illegal BsaI.rc site found at 1263


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Categories
Parameters
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