![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4122020
Designed by: JIAHUA FANG Group: iGEM22_IvyMaker-China (2022-09-17)
SP-MHETase-cMyc-Snooptag+SP-PETase-His-Spytag+SP-CBM-SC-SC-SNC-SC-V5-7813
3 PARTS PFBA1-SP-PETase-His-Spytag-T-ADH2 PFBA1-SP-MHETase-cMyc-Snooptag-T-PGK1 SP-CBM-SC-SC-SNC-SC-V5-7813
In this assembly, SP is signal peptide, the same as last year (BBa_K4122014). GFP is replaced by PETase. His-tag is used for protein purification, and Spytag will form a tight connection with spycatcher, which in turn anchors the attached PETase to the cell surface.Promoter-PFBA1, Terminator-TADH2. RFP is replaced by MHETase. His-tag is used for protein purification, and Snooptag will form a tight connection with snoopcatcher, which in turn anchors the attached MHETase to the cell surface.Promoter-PFBA1, Terminator-T-PGK1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |