Composite

Part:BBa_K4122020

Designed by: JIAHUA FANG   Group: iGEM22_IvyMaker-China   (2022-09-17)
Revision as of 15:43, 17 September 2022 by Registry (Talk | contribs)

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SP-MHETase-cMyc-Snooptag+SP-PETase-His-Spytag+SP-CBM-SC-SC-SNC-SC-V5-7813

3 PARTS PFBA1-SP-PETase-His-Spytag-T-ADH2 PFBA1-SP-MHETase-cMyc-Snooptag-T-PGK1 SP-CBM-SC-SC-SNC-SC-V5-7813

In this assembly, SP is signal peptide, the same as last year (BBa_K4122014). GFP is replaced by PETase. His-tag is used for protein purification, and Spytag will form a tight connection with spycatcher, which in turn anchors the attached PETase to the cell surface.Promoter-PFBA1, Terminator-TADH2. RFP is replaced by MHETase. His-tag is used for protein purification, and Snooptag will form a tight connection with snoopcatcher, which in turn anchors the attached MHETase to the cell surface.Promoter-PFBA1, Terminator-T-PGK1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None