Composite

Part:BBa_K4122020

Designed by: JIAHUA FANG   Group: iGEM22_IvyMaker-China   (2022-09-17)


SP-MHETase-cMyc-Snooptag+SP-PETase-His-Spytag+SP-CBM-SC-SC-SNC-SC-V5-7813

This parts is composed of 3 PARTS:

PFBA1-SP-PETase-His-Spytag-T-ADH2

PFBA1-SP-MHETase-cMyc-Snooptag-T-PGK1

SP-CBM-SC-SC-SNC-SC-V5-7813

When replacing RFP and GFP with MHETase and PETase, we did not observe immunofluorescence with secondary antibodies that should theoretically bind specifically to the V5 tag.

Then we started to analyze.


Fig.1 The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both PETase and MHETase.
Sequence and Features BBa_K4122020 SequenceAndFeatures

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