![](https://parts.igem.org/images/partbypart/icon_cell.png)
Cell
Part:BBa_K4501009:Design
Designed by: Pau Marín Escudero Group: iGEM22_Barcelona_UB (2022-09-09)
CD19-L
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 340 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
Design Notes
Sequence was codon optimized and domesticated to eliminate BsmBI and AarI recognition sites.
Source
Uckun FM, Sun L, Qazi S, Ma H, Ozer Z. Recombinant human CD19-ligand protein as a potent anti-leukaemic agent. Br J Haematol. 2011 Apr;153(1):15-23. doi: 10.1111/j.1365-2141.2011.08583.x. Epub 2011 Feb 17. PMID: 21323891.