Cell

Part:BBa_K4501009:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-09)
Revision as of 06:23, 9 September 2022 by Registry (Talk | contribs)

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CD19-L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


Design Notes

Sequence was codon optimized and domesticated to eliminate BsmBI and AarI recognition sites.


Source

Uckun FM, Sun L, Qazi S, Ma H, Ozer Z. Recombinant human CD19-ligand protein as a potent anti-leukaemic agent. Br J Haematol. 2011 Apr;153(1):15-23. doi: 10.1111/j.1365-2141.2011.08583.x. Epub 2011 Feb 17. PMID: 21323891.

References