Cell

Part:BBa_K4501009:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-09)


CD19-L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


Design Notes

CD19-L (from Uckun FM et al.) was run an homology test to define the propper structure. After that, cytosolic and transmembrane domains were removed to get the active domain. Docking analysis confirmed that the resultant protein is a strong ligand for CD19. Sequence was codon optimized and domesticated to eliminate BsmBI, BbsI, BsaI and AarI recognition sites and RCF10 ilegal sites.

Dockgood.jpg Figure 2. Interaction (docking) of CD19-L (green) and CD19 (yellow).


Source

Uckun FM, Sun L, Qazi S, Ma H, Ozer Z. Recombinant human CD19-ligand protein as a potent anti-leukaemic agent. Br J Haematol. 2011 Apr;153(1):15-23. doi: 10.1111/j.1365-2141.2011.08583.x. Epub 2011 Feb 17. PMID: 21323891.

References