Terminator

Part:BBa_K4390001

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-05)
Revision as of 14:45, 5 September 2022 by Maarten vandenancker (Talk | contribs)


L2U2H09 Terminator

L2U2H09 is a synthetic terminator. The origin is https://www.addgene.org/127042/


L2U2H09 Structure.png

L2U2H09 structural information

This structure of L2U2H09 was generated using mFold DNA folding Form on the UNAFold Web Server. This structure was the likeliest based on loop free-energy predictions, with Δ G = -11.51.

Loop Free-Energy Decomposition
Structural element δG Information
External loop -0.86 15 ss bases & 1 closing helices
Stack -1.00 External closing pair is a4-t25
Stack -1.44 External closing pair is a5-t24
Stack -2.17 External closing pair is c6-g23
Stack -1.84 External closing pair is g7-c22
Stack -2.24 External closing pair is g8-c21
Stack -1.84 External closing pair is c9-g20
Stack -1.84 External closing pair is c10-g19
Stack -1.28 External closing pair is c11-g18
Helix -13.65 9 base pairs
Hairpin loop 3.00 Closing pair is t12-a17

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None