Part:BBa_K4390001

L2U2H09 Terminator

L2U2H09 is a weak synthetic terminator. It has been shown to function in both Escherichia coli and Bacillus subtilis, with higher transcription termination efficiency in B. subtilis.

L2U2H09 structural information This structure of L2U2H09 was generated using mFold DNA folding Form on the UNAFold Web Server. This structure was the likeliest based on loop free-energy predictions, with ΔG = -11.51. Loop Free-Energy Decomposition Structural element ΔG Information External loop -0.86 15 ss bases & 1 closing helices Stack -1.00 External closing pair is a4-t25 Stack -1.44 External closing pair is a5-t24 Stack -2.17 External closing pair is c6-g23 Stack -1.84 External closing pair is g7-c22 Stack -2.24 External closing pair is g8-c21 Stack -1.84 External closing pair is c9-g20 Stack -1.84 External closing pair is c10-g19 Stack -1.28 External closing pair is c11-g18 Helix -13.65 9 base pairs Hairpin loop 3.00 Closing pair is t12-a17

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]