Composite

Part:BBa_K4390004:Design

Designed by: Maarten van den Ancker, William McKenny   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 11:22, 7 August 2022 by Registry (Talk | contribs)

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merR expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This compound (level 1) part was assembled using JUMP, a type IIS assembly method, so all basic (level 0) parts had to have the correct JUMP fusion sites flanking, as well as BsaI sites for level 1 assembly and BsmBI sites for level 0 assembly.



Source

J23100 Promoter - https://parts.igem.org/Part:BBa_J23100 B0034 RBS - https://parts.igem.org/Part:BBa_B0034 merR Transcriptional Repressor - https://parts.igem.org/Part:BBa_K346001 L2U2H09 Terminator - https://parts.igem.org/Part:BBa_K4390001

References