Plasmid_Backbone
Part:BBa_J428343
Designed by: Marcos Valenzuela-Ortega Group: 2021 Engineering Committee (2022-06-15)
pJUMP29-1C KanR Type IIS Level 1 vector for C modules
OriV 9 (pBBR322/ROP; medium copy number)
JUMP Collection
Plasmid | Name | Origin (Copy) | ABr | Level | Module | Selection | 2022 Distribution Location |
---|---|---|---|---|---|---|---|
BBa_J428341 | pJUMP29-1A | pBBR322 (Medium) | Kanamycin | Level 1 | A | BBa_J428326 | Kit Plate 1 Well 2A |
BBa_J428342 | pJUMP29-1B | pBBR322 (Medium) | Kanamycin | Level 1 | B | BBa_J428326 | Kit Plate 1 Well 2C |
BBa_J428343 | pJUMP29-1C | pBBR322 (Medium) | Kanamycin | Level 1 | C | BBa_J428326 | Kit Plate 1 Well 2E |
BBa_J428344 | pJUMP29-1D | pBBR322 (Medium) | Kanamycin | Level 1 | D | BBa_J428326 | Kit Plate 1 Well 2G |
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2948
Illegal suffix found at 16 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NotI site found at 24
Illegal NotI site found at 2954 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2948
Illegal suffix found at 17 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2948
Plasmid lacks a suffix.
Illegal XbaI site found at 2963
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NgoMIV site found at 1390 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2188
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
origin | pBBR322 |
resistance | kanamycin |